![]() ![]() Regulation of gene expression requires that the transcriptional machinery be precisely and efficiently assembled at specific genomic loci 1, 2, 3. Overall, our work expands the gene activation toolbox for biomedical research. Quantitative imaging illustrated that nascent RNA-directed aTFs could induce the high-density assembly of coactivators at transcription sites, which may explain the larger transcriptional burst size induced by Narta. Moreover, Narta provides better activation potency of some expressed genes than CRISPRa and, when used in combination with CRISPRa, has an enhancing effect on gene activation. Importantly, the activation is reversible, tunable and specific. Using Narta, we demonstrate robust activation of a broad range of exogenous and endogenous genes in various cell types, including zebrafish embryos, mouse and human cells. In contrast to existing methods based on recruiting transcriptional modulators via DNA-binding proteins, we developed a strategy termed Narta ( nascent RNA-guided transcriptional activation) to achieve gene activation by recruiting artificial transcription factors (aTFs) to transcription sites through nascent RNAs of the target gene. "I really hope it spurs a debate over how we prioritize our transportation systems and how we actually make sure the people who are walking to work, to shop, to school or taking transit are actually able to get where they need to go, because we can't be trapping people indoors for months on end when our sidewalks are slippery or having them walk out in traffic," he said.Technologies for gene activation are valuable tools for the study of gene functions and have a wide range of potential applications in bioengineering and medicine. "A lot of people either don't know about bylaw enforcement or have given up on it and that's why we're seeing sidewalks persist for long periods of time without actually being cleared properly," he said.īoos said the group hopes the city will consider their report and he noted TriTAG would be willing to work with the city to do more sidewalk audits. "If the sidewalk is not cleared upon re-inspection, city staff will remove the snow and invoice the resident," the city says.įor people unable to clear their own sidewalks, the city said there are community groups that can provide the service or residents can take part in the city's Snow Angel program, where neighbours are asked to help others in their areas.īut Boos said TriTAG heard from residents that even when a complaint is made, the response can be slow. ![]() Bicycle bike racks make return to downtown Kitchener after outcry."We recognize that even at this relatively low cost, there are some who would question whether city plowing of sidewalks would be as effective or responsive as the status quo," the report said. The group's report recommends doing a pilot project in one neighbourhood to get a sense of if it would help. TriTAG has suggested the city should consider looking at either clearing all snow and say the cost would be about $26.29 per household each year. He noted councillors were concerned about the cost of sidewalk clearing, but "what we didn't have was a debate over whether the cost was actually worth it." Other options to clear snow "Complaints are certainly something that council needs to consider and they're sort of a gauge of how happy people may be with a particular service, but what they don't tell us is how well our sidewalks actually enable people to get around," said Mike Boos, an executive committee member with TriTAG. Waterloo cyclist plows Laurel Trail after LRT construction foils city.The staff report only considered complaints to municipalities, and in looking at the complaints it currently receives about residents not clearing sidewalks, staff estimated it would be about the same as if the city was in charge of snow clearing. ![]() In some cases, sidewalks were not cleared to be bare, but instead became snow-packed. ![]() In a November 2016 report evaluating the municipality's snow clearing options, the city found other municipalities that implemented city-wide sidewalk snow-clearing had inconsistent results. The city doesn't have the funding to plow everyone's sidewalks, the city's website about snow removal said. In Kitchener, the city says staff does outreach each year to remind residents its their responsibility to clear snow and ice from sidewalk. The one street in Waterloo was left out of the analysis in the report, although TriTAG noted it saw similar problems to the streets in Kitchener. This is the list of streets monitored as part of TriTAG's sidewalk audit. ![]()
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